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KMID : 0624620140470090494
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BMB Reports
2014 Volume.47 No. 9 p.494 ~ p.499
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Role of NADH: quinone oxidoreductase-1 in the tight junctions of colonic epithelial cells
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Nam Seung-Taek
Hwang Jung-Hwan
Kim Dae-Hong
Park Mi-Jung
Lee Ik-Hwan
Nam Hyo-Jung
Kang Jin-Ku
Kim Sung-Kuk
Hwang Jae-Sam
Chung Hyo-Kyun
Shong Min-Ho
Lee Chul-Ho
Kim Ho
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Abstract
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NADH:quinone oxidoreductase 1 (NQO1) is known to be involved in the regulation of energy synthesis and metabolism, and the functional studies of NQO1 have largely focused on metabolic disorders. Here, we show for the first time that compared to NQO1-WT mice, NQO1-KO mice exhibited a marked increase of permeability and spontaneous inflammation in the gut. In the DSS-induced colitis model, NQO1-KO mice showed more severe inflammatory responses than NQO1-WT mice. Interestingly, the transcript levels of claudin and occludin, the major tight junction molecules of gut epithelial cells, were significantly decreased in NQO1-KO mice. The colons of NQO1-KO mice also showed high levels of reactive oxygen species (ROS) and histone deacetylase (HDAC) activity, which are known to affect transcriptional regulation. Taken together, these novel findings indicate that NQO1 contributes to the barrier function of gut epithelial cells by regulating the transcription of tight junction molecules.
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KEYWORD
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Barrier dysfunction of epithelial cells, Chromosome condensation, Claudin-1, Gut epithelial cell tight junction, Gut inflammation, Histone acetylation/deacetylation, NQO1 knockout mice, Occludin, Transcription
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